Overview

  • Product name
  • Description
    Goat polyclonal to ARPC2
  • Specificity
    This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.

  • Positive control
    • Hela and Human Muscle lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with Human ARPC2 peptide (ab23207).
ICC/IF Use at an assay dependent concentration. PubMed: 22021442

Target

  • Function
    Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
  • Sequence similarities
    Belongs to the ARPC2 family.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin related protein 2/3 complex subunit 2 34kDa antibody
    • Actin related protein 2/3 complex subunit 2 antibody
    • Actin-related protein 2/3 complex subunit 2 antibody
    • ARC 34 antibody
    • ARC34 antibody
    • Arp2/3 complex 34 kDa subunit antibody
    • ARP2/3 protein compex subunit 34 antibody
    • ARPC 2 antibody
    • Arpc2 antibody
    • ARPC2_HUMAN antibody
    • p34 Arc antibody
    • p34-ARC antibody
    • p34Arc antibody
    • PNAS 139 antibody
    • PNAS139 antibody
    • PRO 2446 antibody
    • PRO2446 antibody
    see all

Images

  • ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6).

References

This product has been referenced in:
  • Xia P  et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). Read more (PubMed: 25225459) »
  • Chauhan BK  et al. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108:18289-94 (2011). ICC/IF ; Mouse . Read more (PubMed: 22021442) »

See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab11798.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up