Overview

  • Product name
  • Description
    Rabbit polyclonal to ARPC2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 14-277 of Human ARPC2 (NP_005722).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH 3T3 whole cell lysates. HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
  • Sequence similarities
    Belongs to the ARPC2 family.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin related protein 2/3 complex subunit 2 34kDa antibody
    • Actin related protein 2/3 complex subunit 2 antibody
    • Actin-related protein 2/3 complex subunit 2 antibody
    • ARC 34 antibody
    • ARC34 antibody
    • Arp2/3 complex 34 kDa subunit antibody
    • ARP2/3 protein compex subunit 34 antibody
    • ARPC 2 antibody
    • Arpc2 antibody
    • ARPC2_HUMAN antibody
    • p34 Arc antibody
    • p34-ARC antibody
    • p34Arc antibody
    • PNAS 139 antibody
    • PNAS139 antibody
    • PRO 2446 antibody
    • PRO2446 antibody
    see all

Images

  • ab96779 at 1/200 dilution staining ARPC2 in paraformaldehyde-fixed HeLa cells by immunofluorescence. The lower image shows ab96779 merged with a DNA probe.
  • Anti-ARPC2 antibody (ab96779) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 34 kDa
  • All lanes : Anti-ARPC2 antibody (ab96779) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa

References

This product has been referenced in:
  • Qian X  et al. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology 154:1907-20 (2013). Rat . Read more (PubMed: 23546604) »
  • Qian X  et al. Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis. Am J Physiol Endocrinol Metab 304:E757-69 (2013). WB, IF . Read more (PubMed: 23403943) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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