Overview

  • Product name
  • Description
    Goat polyclonal to ARPC4
  • Specificity
    ab80502 is expected to recognise isoform a (NP_005709.1).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-ERHNKPEVEVR

    , corresponding to internal sequence amino acids 31 - 41 of Human ARPC4 (NP_005709.1)

  • Positive control
    • Human lymph node lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Peptide ELISA: antibody detection limit dilution 1/64000.

WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 20 kDa.

Target

  • Function
    Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
  • Sequence similarities
    Belongs to the ARPC4 family.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin related protein 2/3 complex subunit 4 antibody
    • Actin related protein 2/3 complex subunit 4 20 kD antibody
    • Actin related protein 2/3 complex subunit 4 20kDa antibody
    • Actin related protein 2/3 complex, 20-KD subunit antibody
    • Actin-related protein 2/3 complex subunit 4 antibody
    • ARC20 antibody
    • Arp2/3 complex 20 kDa subunit antibody
    • Arp2/3 protein complex subunit p20 antibody
    • ARPC 4 antibody
    • ARPC4 antibody
    • ARPC4_HUMAN antibody
    • MGC13544 antibody
    • OTTHUMP00000165252 antibody
    • OTTHUMP00000207520 antibody
    • p20 Arc antibody
    • p20-ARC antibody
    • p20ARC antibody
    see all

Images

  • Anti-ARPC4 antibody (ab80502) at 0.03 µg/ml + human lymph node lysate (in RIPA buffer) at 35 µg

    Predicted band size : 20 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.

References

This product has been referenced in:
  • Fu X  et al. Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons. J Neurosci 33:709-21 (2013). Read more (PubMed: 23303949) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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