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Rabbit polyclonal to ARPP21
Detects endogenous levels of total ARPP21 protein.
Predicted to work with:
Synthesized peptide derived from internal sequence of Human ARPP21.
Human heart tissue, HeLa cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
WB: 1/500 - 1/1000. Predicted molecular weight: 89 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Isoform 2 may act as a competitive inhibitor of calmodulin-dependent enzymes such as calcineurin in neurons.
Isoform 2 is expressed in brain. Isoform 1 is present in immature thymocytes (at protein level).
Contains 1 R3H domain.
Phosphorylation at Ser-56 favors interaction with CALM1.
Isoform 1 is methylated by CARM1 in immature thymocytes.
Information by UniProt
Western blot - ARPP21 antibody (ab92647)
All lanes : Anti-ARPP21 antibody (ab92647) at 1/500 dilution Lane 1 : HeLa cell lysate in absence of blocking peptide Lane 2 : HeLa cell lysate in presence of blocking peptide (10 µg) Lysates/proteins at 30 µg per lane. Predicted band size : 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARPP21 antibody (ab92647)
ab92647 at 1/50 dilution staining ARPP21 in (1) Human heart tissue minus blocking peptide and (2) Human heart tissue plus blocking peptide by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"