Overview

  • Product name
  • Description
    Rabbit polyclonal to ARPP21
  • Specificity
    Detects endogenous levels of total ARPP21 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from internal sequence of Human ARPP21.

  • Positive control
    • Human heart tissue, HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/20,000.
    IHC-P: 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
    WB: 1/500 - 1/1000. Predicted molecular weight: 89 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Isoform 2 may act as a competitive inhibitor of calmodulin-dependent enzymes such as calcineurin in neurons.
    • Tissue specificity
      Isoform 2 is expressed in brain. Isoform 1 is present in immature thymocytes (at protein level).
    • Sequence similarities
      Contains 1 R3H domain.
    • Post-translational
      modifications
      Phosphorylation at Ser-56 favors interaction with CALM1.
      Isoform 1 is methylated by CARM1 in immature thymocytes.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0710001E13Rik antibody
      • AI853636 antibody
      • ARP21_HUMAN antibody
      • ARPP-21 antibody
      • ARPP21 antibody
      • cAMP regulated phosphoprotein, 21kDa antibody
      • cAMP-regulated phosphoprotein 21 antibody
      • cAMP-regulated phosphoprotein, 21-KD antibody
      • Cyclic AMP-regulated phosphoprotein antibody
      • Cyclic AMP-regulated phosphoprotein, 21 antibody
      • D9Bwg1012e antibody
      • FLJ32997 antibody
      • MGC109368 antibody
      • OTTHUMP00000161358 antibody
      • OTTHUMP00000208635 antibody
      • OTTHUMP00000208636 antibody
      • OTTHUMP00000208637 antibody
      • OTTHUMP00000208638 antibody
      • OTTHUMP00000208639 antibody
      • OTTHUMP00000208640 antibody
      • OTTHUMP00000208641 antibody
      • OTTHUMP00000208646 antibody
      • OTTHUMP00000208648 antibody
      • OTTHUMP00000208651 antibody
      • OTTHUMP00000208652 antibody
      • OTTHUMP00000208653 antibody
      • OTTHUMP00000208657 antibody
      • Ppp1r1c antibody
      • Ppp1r1cl antibody
      • R3H domain containing 3 antibody
      • R3HDM3 antibody
      • RCS antibody
      • Regulator of calmodulin signaling antibody
      • RGD1307208 antibody
      • RGD1307215 antibody
      • Tarpp antibody
      • Thymocyte cAMP-regulated phosphoprotein antibody
      see all

    Images

    • All lanes : Anti-ARPP21 antibody (ab92647) at 1/500 dilution

      Lane 1 : HeLa cell lysate in absence of blocking peptide
      Lane 2 : HeLa cell lysate in presence of blocking peptide (10 µg)

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 89 kDa
    • ab92647 at 1/50 dilution staining ARPP21 in (1) Human heart tissue minus blocking peptide and (2) Human heart tissue plus blocking peptide by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    References

    ab92647 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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