Overview

  • Product nameAnti-ARRDC3 antibody
    See all ARRDC3 primary antibodies
  • Description
    Rabbit polyclonal to ARRDC3
  • Specificityab64817 detects endogenous levels of total ARRDC3 protein.
  • Tested applicationsSuitable for: ICC/IF, IP, WB, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from the C-terminal of human ARRDC3.

  • Positive control
    • Extracts from Jurkat and COLO205 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab64817 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 25086961
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20603614
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceThe arrestins are a family of proteins that are important for regulating signal transduction within cells. Arrestins are part of a conserved two step mechanism for regulating the activity of G-protein coupled receptors (GPCRs). In response to a stimulus, GPCRs activate a heterotrimeric G protein. In order to turn off this response, or adapt to a constant stimulus, activated receptors need to be silenced. The first step is phosphorylation by a class of serine/threonine kinases called G protein coupled receptor kinases (GRKs). This phosphorylation specifically marks the activated receptor for arrestin binding. Once arrestin is bound to the receptor it is unable to signal further. Recent research continues to expand the known actions of arrestins, which can bind to other classes of receptors and can directly activate signaling pathways on their own. Different arrestins (visual arrestin (or Arrestin 1), beta-arrestin 1 (or Arrestin 2) and beta-arrestin 2 (or Arrestin 3) can reduce the activity of their target GPCRs in several different ways.
  • Cellular localizationCytoplasm. Note: Associated with plasma membrane, as well as with endodomes and lysosomes during endocytosis.
  • Database links
  • Alternative names
    • ARRDC 3 antibody
    • Arrestin domain containing 3 antibody
    • Arrestin domain containing protein 3 antibody
    • KIAA1376 antibody
    • TBP-2-like inducible membrane protein antibody
    • Thioredoxin binding protein 2 like inducible membrane antibody
    • TLIMP antibody
    see all

Anti-ARRDC3 antibody images

  • All lanes : Anti-ARRDC3 antibody (ab64817) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • All lanes : Anti-ARRDC3 antibody (ab64817) at 1/500 dilution

    Lane 1 : Rat brain lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Mouse brain lysates with immunizing peptide at 5 µg


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • IHC image of ab64817 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64817, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab64817 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64817, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ARRDC3 antibody (ab64817)

This product has been referenced in:
  • Soung YH  et al. Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells. Sci Rep 4:3846 (2014). WB ; Human . Read more (PubMed: 24457910) »
  • Noh H  et al. Prediction of miRNA-mRNA associations in Alzheimer's disease mice using network topology. BMC Genomics 15:644 (2014). WB, IP ; Mouse . Read more (PubMed: 25086961) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"