Overview

  • Product name
  • Description
    Rabbit polyclonal to ARRDC4
  • Tested applications
    Suitable for: WB, Indirect ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human ARRDC4.

  • Positive control
    • extracts from COLO cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Indirect ELISA 1/10000.
ICC/IF Use at an assay dependent concentration. PubMed: 23236378

Target

Images

  • All lanes : Anti-ARRDC4 antibody (ab74265) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,36 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Shea FF  et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012). ICC/IF ; Human . Read more (PubMed: 23236378) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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