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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Use at an assay dependent concentration. PubMed: 23236378
Anti-ARRDC4 antibody images
Western blot - ARRDC4 antibody (ab74265)
All lanes :
Anti-ARRDC4 antibody (ab74265) at 1/500 dilution
Lane 1 :
extracts from COLO cells
Lane 2 :
extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size :
Observed band size :
47 kDa (
why is the actual band size different from the predicted?
Additional bands at :
28 kDa,36 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ARRDC4 antibody (ab74265)
This product has been referenced in:
et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012).
Read more (PubMed: 23236378) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"