The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
FunctionDisplays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate.
Tissue specificityWidely expressed, with very low expression in brain, lung, heart and skeletal muscle.
Sequence similaritiesBelongs to the sulfatase family.
Post-translational modificationsN-glycosylated. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. Glycosylated.
Cellular localizationLysosome. Previously observed endoplasmic reticulum localization is most likely due to folding/maturation problems for overexpressed proteins.