Recombinant
RabMAb

Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)

Overview

  • Product name
    Anti-ART3 antibody [EPR7220(2)] - C-terminal
  • Description
    Rabbit monoclonal [EPR7220(2)] to ART3 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ART3 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13508

  • Positive control
    • Human testis tissue; Human testis lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR7220(2)
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab192870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41, 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/20.

Target

  • Tissue specificity
    Testis specific.
  • Sequence similarities
    Belongs to the Arg-specific ADP-ribosyltransferase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosyltransferase 3 antibody
    • ART 3 antibody
    • Art3 antibody
    • Ecto-ADP-ribosyltransferase 3 antibody
    • FLJ26404 antibody
    • mono ADP ribosyltransferase antibody
    • Mono(ADP-ribosyl)transferase 3 antibody
    • NAD(P)(+)--arginine ADP-ribosyltransferase 3 antibody
    • NAR3_HUMAN antibody
    • OTTHUMP00000160603 antibody
    • OTTHUMP00000160604 antibody
    • OTTHUMP00000219067 antibody
    • OTTHUMP00000219069 antibody
    • OTTHUMP00000219070 antibody
    • TMART antibody
    see all

Images

  • Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution + Human testis tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 41 kDa (possible mature (processed) protein).
  • Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody

    Note: The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG  was used as secondary. Counterstain: Hematoxylin. Negative control also shown.

References

ab192870 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192870.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up