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Anti-ART3 antibody [EPR7220(2)] - C-terminal
Rabbit monoclonal [EPR7220(2)] to ART3 - C-terminal
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ART3 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q13508
Human testis tissue; Human testis lysate
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 41, 44 kDa (predicted molecular weight: 44 kDa).
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Belongs to the Arg-specific ADP-ribosyltransferase family.
Information by UniProt
ADP ribosyltransferase 3 antibody
ART 3 antibody
Western blot - Anti-ART3 antibody [EPR7220(2)] (ab192870)
Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution + Human testis tissue lysate at 20 µg
Secondary Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution Predicted band size : 44 kDa Observed band size : 44 kDa Additional bands at : 41 kDa (possible mature (processed) protein).
Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] (ab192870)
Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody
Note: The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] (ab192870)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin. Negative control also shown.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"