Recombinant
RabMAb

Anti-Artemin antibody [EPR9763] (ab178434)

Overview

  • Product name
    Anti-Artemin antibody [EPR9763]
    See all Artemin primary antibodies
  • Description
    Rabbit monoclonal [EPR9763] to Artemin
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Artemin aa 150 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5T4W7

  • Positive control
    • Fetal brain, liver and kidney tissue lysates, T47D cell line lysate; Human cervical carcinoma tissue and Human heart tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9763
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab178434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
    • Tissue specificity
      Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain.
    • Sequence similarities
      Belongs to the TGF-beta family. GDNF subfamily.
    • Developmental stage
      Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Artemin antibody
      • Artn antibody
      • ARTN_HUMAN antibody
      • Enovin antibody
      • EVN antibody
      • NBN antibody
      • Neublastin antibody
      • Neurotrophic factor antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from (1)  fetal kidney lysate or (2) 1XPBS (negative control) using ab178434 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling Artemin with ab178434 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling Artemin with ab178434 at 1/50.

    • All lanes : Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : T47D cell line lysate
      Lane 3 : Fetal kidney lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 23 kDa

    References

    ab178434 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab178434.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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