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Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human Artemis.
(Peptide available as ab35688.)
Our Abpromise guarantee covers the use of ab35649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 78 kDa).
Abcam recommends blocking and incubation using 3% milk.
|ICC/IF||Use a concentration of 1 µg/ml.|
Abcam recommends using milk as the blocking agent. ab35649 detects a strong band at 60 kDa in HepG2 whole cell lysate. It is thought that this band corresponds to isoforms 2 and 3 which have a predicted molecular weight of 65 kDa (SwissProt data).