• Product nameAnti-Artemis antibody
    See all Artemis primary antibodies
  • Description
    Goat polyclonal to Artemis
  • SpecificityThis antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express artemis, it has been tested in the CJ cell line (see below) and in an hTERT line.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 478-490 of Human Artemis.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab3834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).


  • FunctionRequired for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
  • Tissue specificityUbiquitously expressed, with highest levels in the kidney, lung, pancreas and placenta (at the mRNA level). Expression is not increased in thymus or bone marrow, sites of V(D)J recombination.
  • Involvement in diseaseDefects in DCLRE1C are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.
    Defects in DCLRE1C are the cause of severe combined immunodeficiency Athabaskan type (SCIDA) [MIM:602450]. SCIDA is a variety of RS-SCID caused by a founder mutation in Athabascan-speaking native Americans, being inherited as an autosomal recessive trait with an estimated gene frequency of 2.1% in the Navajo population. Affected individuals exhibit clinical symptoms and defects in DNA repair comparable to those seen in RS-SCID.
    Defects in DCLRE1C are a cause of Omenn syndrome (OS) [MIM:603554]. OS is characterized by severe combined immunodeficiency associated with erythrodermia, hepatosplenomegaly, lymphadenopathy and alopecia. Affected individuals have elevated T-lymphocyte counts with a restricted T-cell receptor (TCR) repertoire. They also generally lack B-lymphocytes, but have normal natural killer (NK) cell function (T+ B- NK+).
  • Sequence similaritiesBelongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
  • Post-translational
    Phosphorylation on undefined residues by PRKDC may stimulate endonucleolytic activity on 5' and 3' hairpins and overhangs. PRKDC must remain present, even after phosphorylation, for efficient hairpin opening. Also phosphorylated by ATM in response to ionizing radiation (IR) and by ATR in response to ultraviolet (UV) radiation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A SCID antibody
    • A SCID protein antibody
    • Artemis protein antibody
    • ASCID antibody
    • DCLRE1C antibody
    • DCLRE1C DNA cross link repair 1C antibody
    • DCLRE1C protein antibody
    • DCLREC1C antibody
    • DCR1C_HUMAN antibody
    • DNA cross link repair 1C antibody
    • DNA cross link repair 1C protein antibody
    • DNA cross-link repair 1C protein antibody
    • FLJ11360 antibody
    • FLJ36438 antibody
    • hSNM1C antibody
    • OTTHUMP00000045150 antibody
    • Protein A-SCID antibody
    • Protein ARTEMIS antibody
    • PSO2 homolog antibody
    • RS SCID antibody
    • SCIDA antibody
    • Severe combined immunodeficiency type a antibody
    • SNM1 homolog C antibody
    • SNM1 like protein antibody
    • SNM1-like protein antibody
    • SNM1C antibody
    see all

Anti-Artemis antibody images

  • All lanes : Anti-Artemis antibody (ab3834) at 2.4 µg/ml

    Lane 1 : AT7BI whole cell lysate
    Lane 2 : MO59K whole cell lysate
    Lane 3 : MO59J whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : CJ whole cell lysate

    Predicted band size : 78 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    CJ is an Artemis deficient cell line and so no band is visible.

  • IHC-P of human spleen using ab3834 at a 1:1000 dilution.  Positive staining of T cells and B lymphocytes is observed.

References for Anti-Artemis antibody (ab3834)

This product has been referenced in:
  • Sallmyr A  et al. Up-regulation of WRN and DNA ligase III{alpha} in chronic myeloid leukemia: consequences for the repair of DNA double strand breaks. Blood : (2008). Read more (PubMed: 18524993) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. We don't sell the CJ cell line; the Western blot image located on the online datasheet was provided for us by Dr Penny Jeggo, University of Sussex. As you seem to be experiencing some difficulty with this antibody, I wou...

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