Overview

  • Product nameAnti-ARTS1 antibody [EPR6069]
    See all ARTS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6069] to ARTS1
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to C-terminal amino acids of Human ARTS1.

  • Positive control
    • K562, HUVEC and A431 cell lysates; Human uterus tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionAminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the peptidase M1 family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A LAP antibody
      • A-LAP antibody
      • Adipocyte derived leucine aminopeptidase antibody
      • Adipocyte-derived leucine aminopeptidase antibody
      • ALAP antibody
      • Aminopeptidase PILS antibody
      • Aminopeptidase regulator of TNFR1 shedding antibody
      • APPILS antibody
      • ARTS 1 antibody
      • ARTS-1 antibody
      • Arts1 antibody
      • Endoplasmic reticulum aminopeptidase 1 antibody
      • Endoplasmic reticulum aminopeptidase associated with antigen processing antibody
      • ERAAP antibody
      • ERAAP1 antibody
      • ERAP 1 antibody
      • Erap1 antibody
      • ERAP1_HUMAN antibody
      • KIAA0525 antibody
      • kiaa0525 protein antibody
      • PILS AP antibody
      • PILS-AP antibody
      • PILSA antibody
      • PILSAP antibody
      • Puromycin insensitive leucyl specific aminopeptidase antibody
      • Puromycin-insensitive leucyl-specific aminopeptidase antibody
      • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody
      • type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody
      • VEGF-induced aminopeptidase antibody
      see all

    Anti-ARTS1 antibody [EPR6069] images

    • All lanes : Anti-ARTS1 antibody [EPR6069] (ab124669) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 2000

      Predicted band size : 107 kDa
    • ab124669, at a 1/50 dilution, staining ARTS1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

    References for Anti-ARTS1 antibody [EPR6069] (ab124669)

    This product has been referenced in:
    • Wang B  et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . Read more (PubMed: 23965983) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"