The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 105 kDa (predicted molecular weight: 96 kDa).
Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
Class E basic helix loop helix protein 76 antibody
Class E basic helix-loop-helix protein 76 antibody
Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
Predicted band size : 96 kDa Observed band size : 96 kDa
Lane 1: Wild type HAP1 whole cell lysate (40 µg) Lane 2: Empty lane Lane 3: Aryl hydrocarbon Receptor (KO) whole cell lysate (40 µg) Lane 4: HeLa whole cell lysate (40 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. Ab190797 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Aryl hydrocarbon Receptor [EPR7119(N)(2)] antibody (ab190797)
All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/5000 dilution
Lane 1 : 293 cell lysate Lane 2 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Aryl hydrocarbon Receptor with ab190797 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®555) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue). Negative controls also shown.