Validated using a knockout cell line
Recombinant
RabMAb

Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

Overview

  • Product name
    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]
    See all Aryl hydrocarbon Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR7119(N)(2)] to Aryl hydrocarbon Receptor
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Aryl hydrocarbon Receptor aa 700-800. The exact sequence is proprietary.
    Database link: P35869

  • Positive control
    • PC3, HeLa, 293 and Caco2 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR7119(N)(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 105 kDa (predicted molecular weight: 96 kDa).
ICC/IF 1/100.

Target

  • Function
    Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • Tissue specificity
    Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localization
    Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

Images



  • Predicted band size : 96 kDa
    Observed band size : 96 kDa

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty lane
    Lane 3: Aryl hydrocarbon Receptor (KO) whole cell lysate (40 µg)
    Lane 4: HeLa whole cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. Ab190797 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 96 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/1000 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 96 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Aryl hydrocarbon Receptor with ab190797 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®555)  was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue). Negative controls also shown.

References

ab190797 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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