Overview

  • Product name
    Anti-Arylsulfatase D antibody
    See all Arylsulfatase D primary antibodies
  • Description
    Goat polyclonal to Arylsulfatase D
  • Specificity
    ab81978 is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-DASNGYRALQWNA

    , corresponding to internal sequence amino acids 108-120 of Human Arylsulfatase D (NP_001660.2; NP_033667.2)

  • Positive control
    • A459 and HepG2 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab81978 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Expressed in the pancreas, kidney, liver, lung, placenta, brain and heart.
    • Sequence similarities
      Belongs to the sulfatase family.
    • Post-translational
      modifications
      The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • arsD antibody
      • ARSD_HUMAN antibody
      • Arylsulfatase D antibody
      • ASD antibody
      • EC 3.1.6 antibody
      • OTTHUMP00000022848 antibody
      • OTTHUMP00000022849 antibody
      see all

    Anti-Arylsulfatase D antibody images

    • All lanes : Anti-Arylsulfatase D antibody (ab81978) at 0.3 µg/ml

      Lane 1 : A459 lysate in RIPA buffer
      Lane 2 : A459 lysate in RIPA buffer with immunising peptide

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Arylsulfatase D antibody (ab81978)

    ab81978 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab81978.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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