Overview

  • Product name
  • Description
    Rabbit polyclonal to ASAH1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of 16 amino acids taken from near the C-terminus of human ASAH1. (EAW63795).

  • Positive control
    • Mouse heart tissue lysate
  • General notes

    Multiple isoforms of ASAH1 are known to exist.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
  • Tissue specificity
    Broadly expressed with highest expression in heart.
  • Involvement in disease
    Defects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
  • Sequence similarities
    Belongs to the acid ceramidase family.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC antibody
    • ACDase antibody
    • Acid CDase antibody
    • Acid ceramidase antibody
    • Acid ceramidase precursor antibody
    • Acid ceramidase subunit beta antibody
    • Acylsphingosine deacylase antibody
    • ASAH 1 antibody
    • ASAH antibody
    • ASAH1 antibody
    • ASAH1_HUMAN antibody
    • FLJ21558 antibody
    • FLJ22079 antibody
    • N acylsphingosine amidohydrolase (acid ceramidase) 1 antibody
    • N acylsphingosine amidohydrolase 1 antibody
    • N acylsphingosine amidohydrolase antibody
    • N-acylsphingosine amidohydrolase antibody
    • N-acylsphingosine deacylase antibody
    • PHP antibody
    • PHP32 antibody
    • Putative 32 kDa heart protein antibody
    • SMAPME antibody
    see all

Anti-ASAH1 antibody images

  • Lane 1 : Anti-ASAH1 antibody (ab74469) at 1 µg/ml
    Lane 2 : Anti-ASAH1 antibody (ab74469) at 2 µg/ml

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Lysates/proteins at 15 µg per lane.


    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ab74469 at 2.5µg/ml staining ASAH1 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of ASAH1 in Human Heart cells using ab74469 at 20 ug/ml.

References for Anti-ASAH1 antibody (ab74469)

This product has been referenced in:
  • Braun F  et al. Altered lipid metabolism in the aging kidney identified by three layered omic analysis. Aging (Albany NY) 8:441-57 (2016). IHC . Read more (PubMed: 26886165) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Adrenal carcinoma)
Specification
Adrenal carcinoma
Treatment
Stable transfection with ASAH1-EGFP or EGFP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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