Overview

  • Product name
    Anti-ASAH3 antibody
  • Description
    Rabbit polyclonal to ASAH3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 141-190 (VNAYALNSIA LHILYIVCQE RKTSNKELRH IEVSVVLWAV LTSWISD) of Human ASAH3 (NP_597999).

  • Positive control
    • HCT15 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids.
  • Tissue specificity
    Mainly expressed in epidermis.
  • Sequence similarities
    Belongs to the alkaline ceramidase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acer1 antibody
    • ACER1_HUMAN antibody
    • Acylsphingosine deacylase 3 antibody
    • Alkaline CDase 1 antibody
    • Alkaline ceramidase 1 antibody
    • Alkaline ceramidase antibody
    • AlkCDase 1 antibody
    • ALKCDase1 antibody
    • ASAH 3 antibody
    • MGC138327 antibody
    • MGC138329 antibody
    • N acylsphingosine amidohydrolase (alkaline ceramidase) 3 antibody
    • N acylsphingosine amidohydrolase 3 alkaline ceramidase antibody
    • N acylsphingosine amidohydrolase 3 antibody
    • N-acylsphingosine amidohydrolase 3 antibody
    see all

Images

  • Anti-ASAH3 antibody (ab113916) at 1 µg/ml + HCT15 cell lysate at 10 µg

    Predicted band size : 31 kDa

References

ab113916 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab113916.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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