Anti-ASAP1 / DDEF1 antibody [7B12] (ab125729)

Overview

  • Product name
    Anti-ASAP1 / DDEF1 antibody [7B12]
    See all ASAP1 / DDEF1 primary antibodies
  • Description
    Mouse monoclonal [7B12] to ASAP1 / DDEF1
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, IP, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 977-1532 of Human ASAP1/ DDEF1 with N-terminal proprietary tag produced in E.coli strain BL-21.

  • Positive control
    • Human HT29 and MDA-MB-231 cell lysates; Rat ASML cells and 1AS pancreatic carcinoma cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab125729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 125 kDa.
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use a concentration of 5 µg/ml.
IP Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
  • Sequence similarities
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain
    The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by SRC.
  • Cellular localization
    Cytoplasm. Membrane. Predominantly cytoplasmic (By similarity). Partially membrane-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody
    • 130 kDa phosphatidylinositol 4 antibody
    • 5-biphosphate-dependent ARF1 GTPase-activating protein antibody
    • ADP ribosylation factor directed GTPase activating protein 1 antibody
    • ADP-ribosylation factor-directed GTPase-activating protein 1 antibody
    • AMAP 1 antibody
    • AMAP1 antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARF GTPase activating protein 1 antibody
    • ARF GTPase-activating protein 1 antibody
    • Arf-GAP with SH3 domain antibody
    • ASAP 1 antibody
    • ASAP1 antibody
    • ASAP1_HUMAN antibody
    • Centaurin beta 4 antibody
    • CentB4 antibody
    • DDEF 1 antibody
    • DDEF1 protein antibody
    • DEF-1 antibody
    • Development and differentiation enhancing factor 1 antibody
    • Development and differentiation-enhancing factor 1 antibody
    • Differentiation-enhancing factor 1 antibody
    • KIAA1249 antibody
    • PAG 2 antibody
    • PAG2 antibody
    • PAP antibody
    • PIP2 dependent ARF1 GAP antibody
    • PIP2-dependent ARF1 GAP antibody
    • ZG 14P antibody
    • ZG14P antibody
    see all

References

ab125729 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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