Overview

  • Product name
    Anti-ASAP1 / DDEF1 antibody
    See all ASAP1 / DDEF1 primary antibodies
  • Description
    Rabbit polyclonal to ASAP1 / DDEF1
  • Specificity
    Reacts with ASAP1a and ASAP1b isoforms.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 1128-1147 of Mouse CENTB4.

  • General notes
    Working dilution samples should be discarded if not used within 12 hours. The antibody is stable for at least 6 months when stored appropriately.

Properties

Applications

Our Abpromise guarantee covers the use of ab11011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Predicted molecular weight: 130 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 18519696
ICC/IF 1/1000.

Target

  • Function
    Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
  • Sequence similarities
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain
    The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by SRC.
  • Cellular localization
    Cytoplasm. Membrane. Predominantly cytoplasmic (By similarity). Partially membrane-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody
    • 130 kDa phosphatidylinositol 4 antibody
    • 5-biphosphate-dependent ARF1 GTPase-activating protein antibody
    • ADP ribosylation factor directed GTPase activating protein 1 antibody
    • ADP-ribosylation factor-directed GTPase-activating protein 1 antibody
    • AMAP 1 antibody
    • AMAP1 antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARF GTPase activating protein 1 antibody
    • ARF GTPase-activating protein 1 antibody
    • Arf-GAP with SH3 domain antibody
    • ASAP 1 antibody
    • ASAP1 antibody
    • ASAP1_HUMAN antibody
    • Centaurin beta 4 antibody
    • CentB4 antibody
    • DDEF 1 antibody
    • DDEF1 protein antibody
    • DEF-1 antibody
    • Development and differentiation enhancing factor 1 antibody
    • Development and differentiation-enhancing factor 1 antibody
    • Differentiation-enhancing factor 1 antibody
    • KIAA1249 antibody
    • PAG 2 antibody
    • PAG2 antibody
    • PAP antibody
    • PIP2 dependent ARF1 GAP antibody
    • PIP2-dependent ARF1 GAP antibody
    • ZG 14P antibody
    • ZG14P antibody
    see all

Images

  • ICC/IF image of ab11011 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11011, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ranganathan S  et al. Loss of EGFR-ASAP1 signaling in metastatic and unresectable hepatoblastoma. Sci Rep 6:38347 (2016). IHC-P ; Human . Read more (PubMed: 27910913) »
  • Curtis J  et al. Susceptibility to tuberculosis is associated with variants in the ASAP1 gene encoding a regulator of dendritic cell migration. Nat Genet 47:523-527 (2015). Read more (PubMed: 25774636) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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