Anti-ASAP1 / DDEF1 antibody (ab12423)

Overview

  • Product nameAnti-ASAP1 / DDEF1 antibody
    See all ASAP1 / DDEF1 primary antibodies
  • Description
    Rabbit polyclonal to ASAP1 / DDEF1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 962-978 of Mouse Centaurin beta 4 (ASAP1). The peptide sequence is identical in mouse ASAP1a and ASAP1b isoforms.

  • Positive control
    • Rat brain and mouse brain extracts (Western blotting). Mouse NIH3T3 fibroblast cells (immunofluorescence).
  • General notesDo not store in frost-free freezers.

Properties

Applications

Our Abpromise guarantee covers the use of ab12423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesIF: 1/2000, this is recommended on mouse NIH3T3 fibroblast cells.
    WB: 1/2000, recommended using rat brain and mouse brain extracts. The antibody detects a 130 kDa doublet band, staining is specifically inhibited with the immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPossesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
    • Sequence similaritiesContains 2 ANK repeats.
      Contains 1 Arf-GAP domain.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • DomainThe PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues by SRC.
    • Cellular localizationCytoplasm. Membrane. Predominantly cytoplasmic (By similarity). Partially membrane-associated.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody
      • 130 kDa phosphatidylinositol 4 antibody
      • 5-biphosphate-dependent ARF1 GTPase-activating protein antibody
      • ADP ribosylation factor directed GTPase activating protein 1 antibody
      • ADP-ribosylation factor-directed GTPase-activating protein 1 antibody
      • AMAP 1 antibody
      • AMAP1 antibody
      • ANK repeat and PH domain-containing protein 1 antibody
      • ARF GTPase activating protein 1 antibody
      • ARF GTPase-activating protein 1 antibody
      • Arf-GAP with SH3 domain antibody
      • ASAP 1 antibody
      • ASAP1 antibody
      • ASAP1_HUMAN antibody
      • Centaurin beta 4 antibody
      • CentB4 antibody
      • DDEF 1 antibody
      • DDEF1 protein antibody
      • DEF-1 antibody
      • Development and differentiation enhancing factor 1 antibody
      • Development and differentiation-enhancing factor 1 antibody
      • Differentiation-enhancing factor 1 antibody
      • KIAA1249 antibody
      • PAG 2 antibody
      • PAG2 antibody
      • PAP antibody
      • PIP2 dependent ARF1 GAP antibody
      • PIP2-dependent ARF1 GAP antibody
      • ZG 14P antibody
      • ZG14P antibody
      see all

    References for Anti-ASAP1 / DDEF1 antibody (ab12423)

    ab12423 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. Regarding ab11010 and ab12423, I noted one difference in the datasheet and it is in the "Species Reactivity". For ab11010 - reacts with human and for ab12423 - reacts with human mouse and rat. Both products have been te...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"