• Product nameAnti-ASAP1 / DDEF1 antibody
    See all ASAP1 / DDEF1 primary antibodies
  • Description
    Mouse monoclonal to ASAP1 / DDEF1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: VQSRDAIQKQ ASEDSNDLTP TLPETPVPLP RKINTGKNKV RRVKTIYDCQ ADNDDELTFI EGEVIIVTGE EDQEWWIGHI EGQPERKGVF PVSFVHILSD, corresponding to amino acids 1030 - 1129 of human ASAP1/ DDEF1 (NP_060952) with a tag

  • Positive control
    • Recombinant human ASAP1 / DDEF1 protein fragment (immunogen); IMR-32 and NIH/3T3 cell lysates.


Associated products


Our Abpromise guarantee covers the use of ab76916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 125 kDa.
ELISA Use at an assay dependent dilution.


  • FunctionPossesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
  • Sequence similaritiesContains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.
  • Post-translational
    Phosphorylated on tyrosine residues by SRC.
  • Cellular localizationCytoplasm. Membrane. Predominantly cytoplasmic (By similarity). Partially membrane-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody
    • 130 kDa phosphatidylinositol 4 antibody
    • 5-biphosphate-dependent ARF1 GTPase-activating protein antibody
    • ADP ribosylation factor directed GTPase activating protein 1 antibody
    • ADP-ribosylation factor-directed GTPase-activating protein 1 antibody
    • AMAP 1 antibody
    • AMAP1 antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARF GTPase activating protein 1 antibody
    • ARF GTPase-activating protein 1 antibody
    • Arf-GAP with SH3 domain antibody
    • ASAP 1 antibody
    • ASAP1 antibody
    • ASAP1_HUMAN antibody
    • Centaurin beta 4 antibody
    • CentB4 antibody
    • DDEF 1 antibody
    • DDEF1 protein antibody
    • DEF-1 antibody
    • Development and differentiation enhancing factor 1 antibody
    • Development and differentiation-enhancing factor 1 antibody
    • Differentiation-enhancing factor 1 antibody
    • KIAA1249 antibody
    • PAG 2 antibody
    • PAG2 antibody
    • PAP antibody
    • PIP2 dependent ARF1 GAP antibody
    • PIP2-dependent ARF1 GAP antibody
    • ZG 14P antibody
    • ZG14P antibody
    see all

Anti-ASAP1 / DDEF1 antibody images

  • Anti-ASAP1 / DDEF1 antibody (ab76916) at 5 µg/ml + immunogen at 0.2 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 125 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-ASAP1 / DDEF1 antibody (ab76916) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • Anti-ASAP1 / DDEF1 antibody (ab76916) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 125 kDa
    Observed band size : 125 kDa

References for Anti-ASAP1 / DDEF1 antibody (ab76916)

ab76916 has not yet been referenced specifically in any publications.

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