• Product nameAnti-ASB10 antibody
    See all ASB10 primary antibodies
  • Description
    Mouse monoclonal to ASB10
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 3-109 of Human ASB10


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab58133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionMay be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 7 ANK repeats.
    Contains 1 SOCS box domain.
  • DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ankyrin repeat and SOCS box containing 10 antibody
    • Ankyrin repeat and SOCS box protein 10 antibody
    • Ankyrin repeat- and SOCS box-containing protein 10 antibody
    • ASB 10 antibody
    • ASB-10 antibody
    • ASB10 antibody
    • ASB10_HUMAN antibody
    • GLC1F antibody
    see all

Anti-ASB10 antibody images

  • Predicted band size : 47 kDa
    ASB10 antibody (ab58133) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • IHC image of ab58133 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58133, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ASB10 antibody (ab58133)

ab58133 has not yet been referenced specifically in any publications.

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