Overview

  • Product nameAnti-ASB12 antibody
    See all ASB12 primary antibodies
  • Description
    Rabbit polyclonal to ASB12
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide, corresponding to internal sequence within amino acids 252-301,

    SQDDKGIALLLQARATPRSLLSQVRLVVRRALCQAGQPQAINQLDIPPML

    , of Human ASB12 (NP_569059)

  • Positive control
    • Fetal Muscle Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:1562500.

Target

Anti-ASB12 antibody images

  • Anti-ASB12 antibody (ab81509) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Muscle Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-ASB12 antibody (ab81509)

ab81509 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"