The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 µg/ml. Do not perform antigen retrieval. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125 °C for 4min and under 90 °C for 45min, cool sample subsequently.
FunctionMay be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityHighest level of expression in skeletal muscle. Also expressed in heart, brain, placenta, liver, kidney and pancreas.
PathwayProtein modification; protein ubiquitination.