Overview

  • Product name
    Anti-ASB9 antibody
  • Description
    Rabbit polyclonal to ASB9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-253 of Human ASB9 (NP_001026909).

  • Positive control
    • HeLaS3, HepG2 and MOLT4 cell lysates for WB; HeLa cells for IF/ICC

Properties

Applications

Our Abpromise guarantee covers the use of ab97918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance
    ASB9 may be a substrate-recognition component of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Database links
    • Alternative names
      • Ankyrin repeat and SOCS box protein 9 antibody
      • ASB-9 antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB9 antibody (ab97918)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB9 antibody (ab97918)

      Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97918 antibody at 1:500 dilution.

    • Western blot - Anti-ASB9 antibody (ab97918)
      Western blot - Anti-ASB9 antibody (ab97918)
      All lanes : Anti-ASB9 antibody (ab97918) at 1/1000 dilution

      Lane 1 : HeLaS3 whole cell lysate
      Lane 2 : HepG2 whole cell lysate
      Lane 3 : MOLT4 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 32 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-ASB9 antibody (ab97918)
      Immunocytochemistry/ Immunofluorescence - Anti-ASB9 antibody (ab97918)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97918 at 1/200 dilution. Lower image merged with DNA probe.

    References

    ab97918 has not yet been referenced specifically in any publications.

    Publishing research using ab97918? Please let us know so that we can cite the reference in this datasheet.

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