Anti-ASC-2 antibody (ab56972)

Overview

  • Product nameAnti-ASC-2 antibody
    See all ASC-2 primary antibodies
  • Description
    Mouse monoclonal to ASC-2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1954-2064 of Human ASC-2

Properties

Applications

Our Abpromise guarantee covers the use of ab56972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe protein encoded by this gene is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone dependent fashion. It is involved in the coactivation of different nuclear receptors, such as steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). It probably functions as a general coactivator, rather than just a nuclear receptor coactivator,and may also be involved in the coactivation of the NFkB pathway (referenced from swissprot).
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • Activating signal cointegrator 2 antibody
      • AIB3 antibody
      • Amplified in breast cancer 3 protein antibody
      • Cancer amplified transcriptional coactivator ASC 2 antibody
      • NCOA6 antibody
      • Nuclear receptor coactivator 6 antibody
      • Nuclear receptor coactivator RAP250 antibody
      • PPAR interacting protein antibody
      • Thyroid hormone receptor binding protein antibody
      see all

    Anti-ASC-2 antibody images



    • Predicted band size : 219 kDa
      ASC-2 antibody (ab56972) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-ASC-2 antibody (ab56972)

    ab56972 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for submitting an Abreview of ab56972. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in an untested species and application (mouse, ICC/IF), we would like t...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cultured Cells (RAW 264.7)
    Specification RAW 264.7
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Username

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted May 22 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Monocytes from Apheresis)
    Specification Monocytes from Apheresis
    Fixative BD Cytofix/Cytoperm 20 min
    Permeabilization Yes - BD Cytofix/Cytoperm 20 min
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Feb 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"