Overview

  • Product nameAnti-ASC1 antibody
    See all ASC1 primary antibodies
  • Description
    Rabbit polyclonal to ASC1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to amino acids 225-275 of Human ASC1 (NP_057297.2)

  • Positive control
    • HeLa whole cell lysate 293T whole cell lysate NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionTranscription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating signal cointegrator 1 antibody
    • ASC 1 antibody
    • ASC-1 antibody
    • HsT17391 antibody
    • Thyroid hormone receptor interactor 4 antibody
    • Thyroid receptor interacting protein 4 antibody
    • Thyroid receptor-interacting protein 4 antibody
    • TR-interacting protein 4 antibody
    • TRIP 4 antibody
    • TRIP-4 antibody
    • Trip4 antibody
    • TRIP4_HUMAN antibody
    • ZC2HC5 antibody
    • Zinc finger C2HC5 type antibody
    see all

Anti-ASC1 antibody images

  • All lanes : Anti-ASC1 antibody (ab70627) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 66 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70627 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was used for Western blotting, analysed using ab70627 at 1µg/ml and detected by chemiluminescence for 10 seconds.

References for Anti-ASC1 antibody (ab70627)

ab70627 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela Cells)
Loading amount 20 µg
Specification Hela Cells
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jun 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"