Overview

  • Product name
    Anti-ASC2 antibody
  • Description
    Rabbit polyclonal to ASC2
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Gerbil
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • HEK-293 cells, mouse spleen, prostate and kidney

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP 1/50 - 1/200.

Target

  • Function
    Associates with apoptosis-associated specklike protein containing a CARD domain (ASC) and modulates its ability to collaborate with pyrin and cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS.
  • Tissue specificity
    Predominantly expressed in monocytes, macrophages and granulocytes.
  • Sequence similarities
    Contains 1 DAPIN domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • PAAD domain only protein antibody
    • PAAD-only protein 1 antibody
    • POP1 antibody
    • PYC1 antibody
    • PYD (pyrin domain) containing 1 antibody
    • PYDC1 antibody
    • PYDC1_HUMAN antibody
    • Pyrin domain containing 1 antibody
    • Pyrin domain-containing protein 1 antibody
    • Pyrin only protein 1 antibody
    • Pyrin-only protein 1 antibody
    see all

Images

  • All lanes : Anti-ASC2 antibody (ab47092) at 1/2000 dilution

    Lane 1 : HEK-293 cells transiently transfected with plasmid encoding ASC2
    Lane 2 : HEK-293 cells transiently transfected with plasmid encoding POP1
    Lane 3 : HEK-293 cells transiently transfected with a negative control plasmid


    Predicted band size : 10 kDa
    Observed band size : 10 kDa
  • Immunhistochemical analysis of ASC2/POP1 expression in mouse spleen using ab47092 at 1:2000. Left and Right images: spleen at low and high magnification, respectively. ASC2/POP1 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
  • Immunhistochemical analysis of ASC2/POP1 expression in mouse using ab47092 at 1:2000. A, prostate tissue from a male mouse prostatitis. B, kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively. ASC2/POP1 staining is seen in the granulocytes

References

ab47092 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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