Overview

  • Product name
  • Description
    Rabbit polyclonal to ASCC3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2152-2202 of Human ASCC3 (NP_006819.2).

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab168810 was affinity purified using an epitope specific to ASCC3 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 251 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Enhances NF-kappa-B, SRF and AP1 transactivation.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the helicase family.
    Contains 2 helicase ATP-binding domains.
    Contains 2 helicase C-terminal domains.
    Contains 3 SEC63 domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating signal cointegrator 1 complex subunit 3 antibody
    • ASC-1 complex subunit p200 antibody
    • ASC-1 complex subunit p200-KD subunit antibody
    • ASC1p200 antibody
    • ascc3 antibody
    • ATP binding 1 antibody
    • B630009I04Rik antibody
    • dJ121G13.4 antibody
    • DJ467N11.1 antibody
    • HELC1_HUMAN antibody
    • HELIC1 antibody
    • Helicase antibody
    • helicase, ATP binding 1 antibody
    • p200 antibody
    • RNA helicase family antibody
    • RNAH antibody
    • RP1-121G13.4 antibody
    • Trip4 complex subunit p200 antibody
    see all

Images

  • All lanes : Anti-ASCC3 antibody (ab168810) at 1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 251 kDa


    Exposure time : 3 minutes
  • Detection of ASCC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168810 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab168810 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168810.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up