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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Purity Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). Abcam recommends using milk as the blocking agent (3%).
Function AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
Tissue specificity Expressed specifically in the extravillous trophoblasts of the developing placenta.
Sequence similarities Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization Nucleus.
Information by UniProt
Achaete scute complex like 2 antibody
Achaete scute homolog 2 antibody
achaete-scute complex-like 2 antibody
Anti-Ascl2 antibody images
Western blot - Anti-Ascl2 antibody (ab116699)
Anti-Ascl2 antibody (ab116699) at 1 µg/ml (Milk blocking - 3%) + Human placenta tissue lysate - total protein ( ab29745) at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 20 kDa Observed band size : 22 kDa ( why is the actual band size different from the predicted?) Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands. Exposure time : 12 minutes
References for Anti-Ascl2 antibody (ab116699)
has not yet been referenced specifically in any publications.
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