Overview

  • Product name
  • Description
    Rabbit polyclonal to Ascl2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ascl2.

  • Positive control
    • This antibody gave a positive signal in Human Placenta tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). Abcam recommends using milk as the blocking agent (3%).

Target

  • Function
    AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
  • Tissue specificity
    Expressed specifically in the extravillous trophoblasts of the developing placenta.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Achaete scute complex like 2 antibody
    • Achaete scute homolog 2 antibody
    • achaete-scute complex-like 2 antibody
    • Achaete-scute homolog 2 antibody
    • ASCL2 antibody
    • ASCL2_HUMAN antibody
    • ASH-2 antibody
    • Ash2 antibody
    • bHLHa45 antibody
    • Class A basic helix-loop-helix protein 45 antibody
    • HASH2 antibody
    • Mash2 antibody
    see all

Images

  • Anti-Ascl2 antibody (ab116699) at 1 µg/ml (Milk blocking - 3%) + Human placenta tissue lysate - total protein (ab29745) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

ab116699 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab116699.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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