The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceASCL4 is a basic helix-loop-helix transcription factor. It is essential for the determination of cell fate and the development and differentiation of numerous tissues. It could be a transcriptional regulator involved in skin development.