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Abcam's Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at ? = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
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Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.
|Ascorbic Acid Assay Buffer||WM||1 x 25ml|
|Ascorbic Acid Enzyme Mix (lyophilized)||Green||1 vial|
|Ascorbic Acid Probe (DMSO)||Red||1 x 0.2ml|
|Ascorbic Acid Standard (20 µmole)||Yellow||1 vial|
|Catalyst||Blue||1 x 500µl|
Our Abpromise guarantee covers the use of ab65346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtracted
ab65346 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"