Overview

  • Product nameAscorbic Acid Assay Kit
    See all Ascorbic acid kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Range
    0.01 nmol/well - 10 nmol/well
  • Assay time
    0h 30m
  • Product overview

    Abcam's Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at ? = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceAscorbic Acid (C6H8O6) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals.

Applications

Our Abpromise guarantee covers the use of ab65346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Ascorbic Acid Assay Kit images

  • Standard curve (fluorimetric) :  mean of duplicates (+/-SD) with background readings subtracted

  • Ascorbic Acid Standard Curve.

Protocols

References for Ascorbic Acid Assay Kit (ab65346)

ab65346 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that the kit can be used with strawberry samples.

We recomend following guidelines for lysates preparation;

Homogenize the strawberries to homogeneity using the assay buffer in the kit and then centrifuge at top speed ...

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Thank you for contacting us.

In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. So if the vitamin C is already oxidized, it is l...

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Frozen samples should be thawed at room temperature, then kept on ice and used promptly in the assay (not warmed to room temperature). Ab65346 is recommended for your samples, since they are already diluted and will be further diluted during the assay....

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Thank you for your inquiry.

The lab was able to provide this information:

1 mole/lt is 1 M.
This standard is 20 µmole. You reconstitute it in 200 µl. That is 20 µmole/200 µl (or 100 µmole/ml which ...

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Thank you for calling Abcam earlier today.

I have talked to the lab about whether the presence of EDTA in the samples would affect the kit and they say that it should be ok.

Please let me know if you have any more questions.

I understand your concern. Signal from pre-existing carbonyl groups, before the catalytic generation of  carbonyl from ascorbic acid, would be an issue with other samples types too, including serum and plasma. The background contributed by sample prote...

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Thanks for your call this week and for your patience while I have been in touch with the lab about ab65346. This kit does utilize a reaction on carbonyl groups, so it will likely cross-react with the PVP though this has not been specifically tes...

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Thank you for your inquiry. I just heard back from the testing laboratory that these kits cannot distinguish ascorbic acid isoforms. I am sorry that this information could not be more helpful, but please let us know if we can be of any other help.

Thank you for contacting us. I have hear back from the ab with the following information to your questions: 1. Yes, you can use plasma samples – but for ab65656 we recommend removing the proteins (e.g. by using spin filter (10 kDa cutoff) (ab933...

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Thank you for your inquiry. Of the two kits, ab65656 is more suitable. Theoretically ab65656 should be compatible with plant samples as long as the lysates are prepared in the assay buffer provided in the kit. Technically though, since we have ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"