Overview

  • Product name
  • Description
    Rabbit polyclonal to ASF1 A+B
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Recombinant full length protein (Fruit fly (Drosophila melanogaster))

Properties

Applications

Our Abpromise guarantee covers the use of ab53608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 24 kDa.
ICC/IF 1/200.
IP 1/500.

Target

  • Relevance
    Asf1 (anti silencing function 1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled like kinase (TLK).
  • Cellular localization
    Nuclear
  • Alternative names
    • Anti silencing factor 1 antibody
    • anti-silencing function 1A antibody
    • anti-silencing function 1B antibody
    • ASF1 anti-silencing function 1 homolog A antibody
    • ASF1 anti-silencing function 1 homolog B antibody
    • ASF1 antibody
    • ASF1A antibody
    • ASF1B antibody
    see all

Images

  • All lanes : Anti-ASF1 A+B antibody (ab53608) at 1/2000 dilution

    Lane 1 : Drosophila embryo lysate
    Lane 2 : Drosophila embryo lysate
    Lane 3 : Drosophila embryo lysate


    Predicted band size : 24 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Lario LD  et al. ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis. Plant Physiol 162:1164-77 (2013). Read more (PubMed: 23596192) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.

The link to the Uniprot entry for drosophila ASF1 is as follows:

http://www.uniprot.org/uniprot/Q9V464

I hope this information is helpful to you. Please do not hesitate to contact us if you ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Fetal Lung Fibroblasts)
Specification
Human Fetal Lung Fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - Triton-X 0.3%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted May 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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