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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromosome
Overview
Product name
Anti-ASF1A antibody
See all ASF1A products (5) ...
Description
Rabbit polyclonal to ASF1A
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chicken, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1 alpha/beta.
(Peptide available as ab37490.)
Positive control
ab24171 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line) and MCF-7 (Human breast adenocarcinoma cell line).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/250Detects a band of approximately 23 , 33 kDa (predicted molecular weight: 23 kDa).
Target
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the ASF1 family.
Post-translational
modifications
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y294
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anti silencing function 1A antibody
- Anti-silencing function protein 1 homolog A antibody
- ASF1 anti silencing function 1 homolog A antibody
see all
Database links
- Entrez Gene: 723780 Chicken
- Entrez Gene: 618099 Cow
- Entrez Gene: 25842 Human
- Omim: 609189 Human
- SwissProt: Q2KIG1 Cow
- SwissProt: Q9Y294 Human
- Unigene: 1997 Chicken
- Unigene: 292316 Human
Anti-ASF1A antibody images:
Western blot - ASF1 alpha/beta antibody (ab24171)
All lanes : Anti-ASF1A antibody (ab24171) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23,33 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible non-specific secondary antibody binding).
ASF1 typically shows up as a band at approximately 33 kDa band on Western blots but has a predicted molecular weight of 23kDa. Occasionally doublet or triplet bands can be observed due to phosphorylation differences.
Immunocytochemistry/ Immunofluorescence - ASF1 alpha/beta antibody (ab24171)
ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-ASF1A antibody (ab24171)
ab24171 has not yet been referenced specifically in any publications.
Publishing research using ab24171? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"