The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
Peptide ELISA: antibody detection limit dilution 1:32,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHistone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the ASF1 family.
Post-translational modificationsPhosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.