Overview

  • Product name
  • Description
    Rabbit polyclonal to ASF1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1A.

    (Peptide available as ab71590.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat, Staurosporine-Treated Jurkat (24hr, 500nM)

Properties

Applications

Our Abpromise guarantee covers the use of ab66754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the ASF1 family.
  • Post-translational
    modifications
    Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anti silencing function 1A antibody
    • Anti-silencing function protein 1 homolog A antibody
    • ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
    • ASF1 anti silencing function 1 homolog A antibody
    • asf1a antibody
    • ASF1A_HUMAN antibody
    • CCG1 interacting factor A antibody
    • CCG1-interacting factor A antibody
    • CGI 98 antibody
    • CIA antibody
    • hAsf1 antibody
    • hAsf1a antibody
    • hCIA antibody
    • Histone chaperone ASF1A antibody
    • HSPC146 antibody
    see all

Images

  • All lanes : Anti-ASF1A antibody (ab66754) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 39 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    The 21 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to ASF1a.

References

ab66754 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 15 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymphoma)
Specification
lymphoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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