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Our Abpromise guarantee covers the use of ab50699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||1/50. Detects a band of approximately 75-76 kDa (predicted molecular weight: 69 kDa).|
|Dot blot||Use at an assay dependent concentration.|