Overview

  • Product name
  • Description
    Rabbit polyclonal to ASH2L
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from residues 54-103 of human ASH2L.

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/12500.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
  • Tissue specificity
    Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
  • Sequence similarities
    Contains 1 B30.2/SPRY domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASH 2 antibody
    • Ash2 (absent small or homeotic) like antibody
    • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
    • ASH2 antibody
    • ASH2 LIKE antibody
    • ASH2 like protein antibody
    • ASH2-like protein antibody
    • Ash2l antibody
    • ASH2L_HUMAN antibody
    • ASH2L1 antibody
    • ASH2L2 antibody
    • Bre 2 antibody
    • Bre2 antibody
    • Discs 2-like antibody
    • Drosophila absent, small, or homeotic antibody
    • Drosophila ASH2-like antibody
    • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
    see all

Images

  • Anti-ASH2L antibody (ab28426) at 0.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP-conjugated anti rabbit IgG diluted 1/50,000 - 1/100,000

    Predicted band size : 69 kDa

References

This product has been referenced in:
  • De Santa F  et al. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing. Cell 130:1083-94 (2007). WB ; Human, Mouse . Read more (PubMed: 17825402) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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