Overview

  • Product nameAnti-ASH2L antibody
    See all ASH2L primary antibodies
  • Description
    Rabbit polyclonal to ASH2L
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 288-337 (AAGSSGKGRG AKRKQQDGGT TGTTKKARSD PLFSAQRLPP HGYPLEHPFN) of Human ASH2L (NP_004665).

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
  • Tissue specificityUbiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
  • Sequence similaritiesContains 1 B30.2/SPRY domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASH 2 antibody
    • Ash2 (absent small or homeotic) like antibody
    • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
    • ASH2 antibody
    • ASH2 LIKE antibody
    • ASH2 like protein antibody
    • ASH2-like protein antibody
    • Ash2l antibody
    • ASH2L_HUMAN antibody
    • ASH2L1 antibody
    • ASH2L2 antibody
    • Bre 2 antibody
    • Bre2 antibody
    • Discs 2-like antibody
    • Drosophila absent, small, or homeotic antibody
    • Drosophila ASH2-like antibody
    • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
    see all

Anti-ASH2L antibody images

  • Anti-ASH2L antibody (ab84732) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : approx. 75 kDa. We are unsure as to the identity of these extra bands.
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ASH2HL with ab84732 at 5.0µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ASH2L with ab84732 at 5.0µg/ml.

References for Anti-ASH2L antibody (ab84732)

ab84732 has not yet been referenced specifically in any publications.

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