Recombinant
RabMAb

Anti-ASH2L antibody [EPR13106] (HRP) (ab209317)

Overview

  • Product name
    Anti-ASH2L antibody [EPR13106] (HRP)
    See all ASH2L primary antibodies
  • Description
    Rabbit monoclonal [EPR13106] to ASH2L (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ASH2L aa 50-150. The exact sequence is proprietary.
    Database link: Q9UBL3

  • Positive control
    • WB: HEK293, HeLa, C6 and PC12 whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).

Target

  • Function
    Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
  • Tissue specificity
    Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
  • Sequence similarities
    Contains 1 B30.2/SPRY domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASH 2 antibody
    • Ash2 (absent small or homeotic) like antibody
    • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
    • ASH2 antibody
    • ASH2 LIKE antibody
    • ASH2 like protein antibody
    • ASH2-like protein antibody
    • Ash2l antibody
    • ASH2L_HUMAN antibody
    • ASH2L1 antibody
    • ASH2L2 antibody
    • Bre 2 antibody
    • Bre2 antibody
    • Discs 2-like antibody
    • Drosophila absent, small, or homeotic antibody
    • Drosophila ASH2-like antibody
    • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
    see all

Images

  • All lanes : Anti-ASH2L antibody [EPR13106] (HRP) (ab209317) at 1/3000 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa whole cell lysate (ab150035)
    Lane 3 : C6 (Rat glioma cell line) Whole Cell Lysate
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209317 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209317 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab209317.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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