Recombinant
RabMAb

Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Overview

  • Product name
    Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker
    See all ASH2L primary antibodies
  • Description
    Rabbit monoclonal [EPR13107(B)] to ASH2L - Nuclear Marker
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ASH2L aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UBL3

  • Positive control
    • WB: HeLa, 293T, Jurkat and K562 cell lysates. IHC-P: Human colon and testis tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/1200.
IHC-P 1/50 - 1/100.
WB 1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
    • Tissue specificity
      Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
    • Sequence similarities
      Contains 1 B30.2/SPRY domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASH 2 antibody
      • Ash2 (absent small or homeotic) like antibody
      • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
      • ASH2 antibody
      • ASH2 LIKE antibody
      • ASH2 like protein antibody
      • ASH2-like protein antibody
      • Ash2l antibody
      • ASH2L_HUMAN antibody
      • ASH2L1 antibody
      • ASH2L2 antibody
      • Bre 2 antibody
      • Bre2 antibody
      • Discs 2-like antibody
      • Drosophila absent, small, or homeotic antibody
      • Drosophila ASH2-like antibody
      • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa labeling ASH2Lwith ab176334 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with ab176334 at 1/50 dilution.

    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with ab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • All lanes : Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : Jurkat cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 69 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with ab176334 at 1/50 dilution.

    References

    ab176334 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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