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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ASH2L aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UBL3
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab176334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/100.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 69 kDa.|
Immunofluorescent analysis of HeLa labeling ASH2Lwith ab176334 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with ab176334 at 1/50 dilution.
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with ab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with ab176334 at 1/50 dilution.
ab176334 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"