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Synthetic peptide found within the range of 50 amino acids 99-148 of Human Asialoglycoprotein Receptor 1
Our Abpromise guarantee covers the use of ab42488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23908685|
ab42488 staining Asialoglycoprotein Receptor 1 (red) in rat hepatocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in PBS + 1% donkey serum) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Blue - nucleus (DAPI).