Anti-Asialoglycoprotein Receptor 1 antibody (ab49355)

Overview

  • Product nameAnti-Asialoglycoprotein Receptor 1 antibody
    See all Asialoglycoprotein Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Asialoglycoprotein Receptor 1
  • SpecificityThis antibody recognizes Asialoglycoprotein receptor 1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: GLRETFSNFT ASTEAQVKGL STQGGNVGRK MKSLESQLEK QQKDLSEDHS, corresponding to N terminal amino acids 72-121 of Human Asialoglycoprotein Receptor 1

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • Tissue specificityExpressed exclusively in hepatic parenchymal cells.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Post-translational
    modifications
    Phosphorylated on a cytoplasmic Ser residue.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASGP-R 1 antibody
    • ASGPR 1 antibody
    • ASGPR antibody
    • ASGPR1 antibody
    • Asgr1 antibody
    • ASGR1_HUMAN antibody
    • Asialoglycoprotein receptor 1 antibody
    • C type lectin domain family 4 member H1 antibody
    • C-type lectin domain family 4 member H1 antibody
    • CLEC4H1 antibody
    • Hepatic lectin H1 antibody
    • HL-1 antibody
    see all

Anti-Asialoglycoprotein Receptor 1 antibody images



  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Human 293T cell Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Human Hela Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Hum. Fetal Kidney Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Human Jurkat Lysate


  • Predicted band size : 33 kDa
    Western Blot using ab49355 at 1.0ug/ml. Sample type: Human MCF7 Cell Lysate
  • Anti-Asialoglycoprotein Receptor 1 antibody (ab49355) at 0.6 µg/ml + Fetal liver lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab49355 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49355, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Asialoglycoprotein Receptor 1 antibody (ab49355)

ab49355 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Loading amount 26 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 21 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2 cells)
Permeabilization Yes - 0.2% Triton X-100
Specification HepG2 cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jul 27 2015

Thank you for contacting us.

Immunohistochemistry on frozen sections is the best technique for snap frozen sections. Please click the link below to know about IHC-FR

http://www.abcam.com/index.html?pageconfig=resource&rid=11383...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Specification HepG2
Fixative Paraformaldehyde
Permeabilization No
Blocking step Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 23 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"