Anti-Asialoglycoprotein Receptor 1 antibody (ab88042)

Overview

  • Product name
    Anti-Asialoglycoprotein Receptor 1 antibody
    See all Asialoglycoprotein Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Asialoglycoprotein Receptor 1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Asialoglycoprotein Receptor 1.

    (Peptide available as ab98248.)

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate and FFPE human liver tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 33 kDa).

Target

  • Function
    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • Tissue specificity
    Expressed exclusively in hepatic parenchymal cells.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    Phosphorylated on a cytoplasmic Ser residue.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASGP-R 1 antibody
    • ASGPR 1 antibody
    • ASGPR antibody
    • ASGPR1 antibody
    • Asgr1 antibody
    • ASGR1_HUMAN antibody
    • Asialoglycoprotein receptor 1 antibody
    • C type lectin domain family 4 member H1 antibody
    • C-type lectin domain family 4 member H1 antibody
    • CLEC4H1 antibody
    • Hepatic lectin H1 antibody
    • HL-1 antibody
    see all

Anti-Asialoglycoprotein Receptor 1 antibody images

  • Anti-Asialoglycoprotein Receptor 1 antibody (ab88042) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • IHC image of ab88042 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88042, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Asialoglycoprotein Receptor 1 antibody (ab88042)

This product has been referenced in:
  • Zhang D  et al. Simplified quantification method for in vivo SPECT/CT imaging of asialoglycoprotein receptor with (99m)Tc-p(VLA-co-VNI) to assess and stage hepatic fibrosis in mice. Sci Rep 6:25377 (2016). Read more (PubMed: 27150943) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 13 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up