The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Predicted molecular weight: 59 kDa.
Dilute in PBS or medium which is identical to that used in the assay system. At 100 nM, this antibody inhibits 50% of Factor XII activity. This antibody can also be used in a coagulation assay. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionCation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
Tissue specificityExpressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN3 subfamily.
Developmental stageExpressed in fetal tissues, expression increases in lung and kidney adult tissues.
DomainThe PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1.
Post-translational modificationsPhosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ACCN3/ASIC3-ACCN1/ASIC2b channel, but does not activate a homomeric ACCN3 channel.
Cellular localizationCell membrane. Cytoplasm. Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human kidney tissue labelling ASIC3 with ab49333 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubules. Magnification: 400X.
Western blot - ASIC3 antibody (ab49333)
Anti-ASIC3 antibody (ab49333) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 59 kDa Observed band size : 59 kDa Gel concentration 12%
References for Anti-ASIC3 antibody (ab49333)
This product has been referenced in:
Gilbert HT et al. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target. Sci Rep6:37360 (2016).
Read more (PubMed: 27853274) »