Overview

  • Product name
  • Description
    Rabbit polyclonal to ASIC3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cat, Pig
  • Immunogen

    A region within synthetic peptide: QTALDERESH RLIFPAVTLC NINPLRRSRL TPNDLHWAGS ALLGLDPAEH, corresponding to N terminal amino acids 73-122 of Human ASIC3

  • Positive control
    • Fetal kidney lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab49333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 59 kDa.

Dilute in PBS or medium which is identical to that used in the assay system. At 100 nM, this antibody inhibits 50% of Factor XII activity. This antibody can also be used in a coagulation assay. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
  • Tissue specificity
    Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.
  • Sequence similarities
    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN3 subfamily.
  • Developmental stage
    Expressed in fetal tissues, expression increases in lung and kidney adult tissues.
  • Domain
    The PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1.
  • Post-translational
    modifications
    Phosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ACCN3/ASIC3-ACCN1/ASIC2b channel, but does not activate a homomeric ACCN3 channel.
  • Cellular localization
    Cell membrane. Cytoplasm. Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACCN 3 antibody
    • Accn3 antibody
    • ACCN3_HUMAN antibody
    • Acid sensing ion channel 3 antibody
    • Acid-sensing ion channel 3 antibody
    • Amiloride sensitive cation channel 3 antibody
    • Amiloride sensitive cation channel 3 testis antibody
    • Amiloride-sensitive cation channel 3 antibody
    • ASIC 3 antibody
    • ASIC3 antibody
    • DRASIC antibody
    • hASIC 3 antibody
    • hASIC3 antibody
    • hTNaC 1 antibody
    • hTNaC1 antibody
    • Modulatory subunit of ASIC 2a antibody
    • Modulatory subunit of ASIC2a antibody
    • Neuronal amiloride sensitive cation channel 3 antibody
    • Neuronal amiloride-sensitive cation channel 3 antibody
    • Proton gated cation channel subunit antibody
    • SLNAC 1 antibody
    • SLNAC1 antibody
    • Testis sodium channel 1 antibody
    • TNaC 1 antibody
    • TNaC1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human kidney tissue labelling ASIC3 with ab49333 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubules. Magnification: 400X.

  • Anti-ASIC3 antibody (ab49333) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Gel concentration 12%

References

This product has been referenced in:
  • Gilbert HT  et al. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target. Sci Rep 6:37360 (2016). WB, IF ; Human . Read more (PubMed: 27853274) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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