The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
Use a concentration of 20 µg/ml.
Use a concentration of 2 µg/ml.
FunctionComponent of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Tissue specificityAbundantly expressed in heart and pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Post-translational modificationsDephosphorylated and activated by PGAM5.
Immunofluorescence of ASK1 in 3T3 cells using ab62575 at 20 ug/ml.
References for Anti-ASK1 antibody (ab62575)
This product has been referenced in:
Pepin E et al. Deletion of apoptosis signal-regulating kinase 1 (ASK1) protects pancreatic beta-cells from stress-induced death but not from glucose homeostasis alterations under pro-inflammatory conditions. PLoS One9:e112714 (2014).
Read more (PubMed: 25383781) »