Overview

  • Product nameAnti-ASK1 antibodySee all ASK1 primary antibodies ...
  • Description
    Rabbit polyclonal to ASK1
  • Tested applicationsWB, ICC/IF, ICC more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human ASK1

  • Positive control
    • SW1353 cell lysate and A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 2 µg/ml.

Target

  • FunctionComponent of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • Tissue specificityAbundantly expressed in heart and pancreas.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dephosphorylated and activated by PGAM5.
  • Target information above from: UniProt accession Q99683 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis signal regulating kinase 1 antibody
    • Apoptosis signal-regulating kinase 1 antibody
    • ASK 1 antibody
    • ASK-1 antibody
    • ASK1 antibody
    • M3K5 antibody
    • M3K5_HUMAN antibody
    • MAP/ERK kinase kinase 5 antibody
    • MAP3K5 antibody
    • MAPK/ERK kinase kinase 5 antibody
    • MAPKKK5 antibody
    • MEK kinase 5 antibody
    • MEKK 5 antibody
    • MEKK5 antibody
    • Mitogen activated protein kinase kinase kinase 5 antibody
    • Mitogen-activated protein kinase kinase kinase 5 antibody
    see all

Anti-ASK1 antibody images

  • ab62575 at 2µg/ml staining ASK1 in A431 cells by Immunocytochemistry.
  • Lane 1 : Anti-ASK1 antibody (ab62575) at 0.5 µg/ml
    Lane 2 : Anti-ASK1 antibody (ab62575) at 1 µg/ml
    Lane 3 : Anti-ASK1 antibody (ab62575) at 2 µg/ml

    Lane 1 : SW1353 cell lysate
    Lane 2 : SW1353 cell lysate
    Lane 3 : SW1353 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 155 kDa
    Observed band size : 155 kDa
  • Immunofluorescence of ASK1 in 3T3 cells using ab62575 at 20 ug/ml.

References for Anti-ASK1 antibody (ab62575)

ab62575 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (neuron)
Loading amount 20 µg
Specification neuron
Treatment ask1 SiRNA
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Zhihui

Verified customer

Submitted Apr 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"