General notesab94062 is a 293T cell transfected lysate in which Human ASK1 has been transiently over-expressed using a pCMV-ASK1 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed how the transfected lysate was prepared view preparation notes
BackgroundFunction: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Tissue specificity: Abundantly expressed in heart and pancreas.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
PTM: Dephosphorylated and activated by PGAM5.