ASK1 overexpression 293T lysate (whole cell) (ab94062)

Overview

  • Product name
    ASK1 overexpression 293T lysate (whole cell)
  • Description
    ASK1 overexpression lysate
  • General notes
    ab94062 is a 293T cell transfected lysate in which Human ASK1 has been transiently over-expressed using a pCMV-ASK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% SDS, 0.788% Tris HCl, 10% Glycerol
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Tissue specificity: Abundantly expressed in heart and pancreas. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. PTM: Dephosphorylated and activated by PGAM5.

Applications

Our Abpromise guarantee covers the use of ab94062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94062 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ASK1 antibody (ab55704) at 1/500 dilution

    Lane 1 : ASK1 293T Transfected Lysate - Positive Control (ab94061)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94062 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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