Anti-ASK1 (phospho S966) antibody (ab39402)

Overview

  • Product name
    Anti-ASK1 (phospho S966) antibody
    See all ASK1 primary antibodies
  • Description
    Rabbit polyclonal to ASK1 (phospho S966)
  • Specificity
    ab39402 detects endogenous levels of ASK1 only when phosphorylated at Serine 966.
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide: SISpLP, derived from Human ASK1 around the phosphorylation site of Serine 966

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab39402 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • Tissue specificity
    Abundantly expressed in heart and pancreas.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dephosphorylated and activated by PGAM5.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis signal regulating kinase 1 antibody
    • Apoptosis signal-regulating kinase 1 antibody
    • ASK 1 antibody
    • ASK-1 antibody
    • ASK1 antibody
    • M3K5 antibody
    • M3K5_HUMAN antibody
    • MAP/ERK kinase kinase 5 antibody
    • MAP3K5 antibody
    • MAPK/ERK kinase kinase 5 antibody
    • MAPKKK5 antibody
    • MEK kinase 5 antibody
    • MEKK 5 antibody
    • MEKK5 antibody
    • Mitogen activated protein kinase kinase kinase 5 antibody
    • Mitogen-activated protein kinase kinase kinase 5 antibody
    see all

Images

  • ab39402, at a 1/100 dilution, staining human ASK1 in breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.
  • ab39402, at a 1/100 dilution, staining human ASK1 in breast carcinoma tissue (treated with synthesized phosphopeptide) by Immunohistochemistry, Paraffin embedded tissue.

References

ab39402 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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