Overview

  • Product name
  • Description
    Rabbit polyclonal to ASMTL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ASMTL aa 230-259 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O95671

  • Positive control
    • MDA-MB23, HL-60, Hela, A549 and CEM cell line lysates. Human colon tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171622 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 69 kDa.

Target

  • Function
    Unknown. The presence of the putative catalytic domain of S-adenosyl-L-methionine binding argues for a methyltransferase activity.
  • Tissue specificity
    Widely expressed. In adult, highly expressed in pancreas, placenta, fibroblast, thymus, prostate, testis, ovary and colon. Expressed at lower levels in spleen, small intestine and leukocytes. In fetus, expressed at high levels in the lung and kidney and at lower level in brain and liver.
  • Sequence similarities
    In the N-terminal section; belongs to the maf family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylserotonin N methyltransferase like antibody
    • Acetylserotonin O methyltransferase like antibody
    • ASML_HUMAN antibody
    • ASMTL antibody
    • ASMTLX antibody
    • ASMTLY antibody
    • ASTML antibody
    • N acetylserotonin O methyltransferase like protein antibody
    • N-acetylserotonin O-methyltransferase-like protein antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human colon tissue labeling ASMTL with ab171622 at 1/10 dilution,  followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-ASMTL antibody (ab171622) at 1/100 dilution

    Lane 1 : MDA-MB231 cell line lysate
    Lane 2 : HL-60 cell line lysate
    Lane 3 : Hela cell line lysate
    Lane 4 : A549 cell line lysate
    Lane 5 : CEM cell line lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 69 kDa

References

ab171622 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171622.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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