Overview

  • Product name
  • Description
    Rabbit polyclonal to ASPA
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-313 of Human ASPA (Uniprot ID P45381).

  • Positive control
    • Mouse brain whole cell lysate. HeLa cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
  • Tissue specificity
    Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver.
  • Involvement in disease
    Defects in ASPA are the cause of Canavan disease (CAND) [MIM:271900]; also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average.
  • Sequence similarities
    Belongs to the AspA/AstE family. Aspartoacylase subfamily.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACY 2 antibody
    • ACY-2 antibody
    • ACY2 antibody
    • ACY2_HUMAN antibody
    • Aminoacylase 2 antibody
    • Aminoacylase-2 antibody
    • Aminoacylase2 antibody
    • ASP antibody
    • ASPA antibody
    • Aspartoacylase (aminoacylase 2, Canavan disease) antibody
    • Aspartoacylase (Canavan disease) antibody
    • Aspartoacylase antibody
    • NUR 7 antibody
    • NUR7 antibody
    • OTTMUSP00000006437 antibody
    • RP23-213I10.1 antibody
    • Small lethargic antibody
    see all

Images

  • Anti-ASPA antibody (ab154503) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg/ml

    Predicted band size : 36 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling ASPA using ab154503 antibody at 1/500 dilution. Lower panel shows costaining with Hoechst 33342.

References

This product has been referenced in:
  • Carpinelli MR  et al. A new mouse model of Canavan leukodystrophy displays hearing impairment due to central nervous system dysmyelination. Dis Model Mech 7:649-57 (2014). WB ; Mouse . Read more (PubMed: 24682784) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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