Asparaginase Activity Assay Kit (ab107922)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Assay time: 40 min
- Sample type: Cell Lysate
Overview
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Product name
Asparaginase Activity Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell Lysate -
Assay type
Enzyme activity -
Assay time
0h 40m -
Product overview
Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.
In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
Asparaginase assay protocol summary:
- add reaction mix to sample and standard wells, mix and incubate for 10 min
- analyze with microplate reader in kinetic mode for 30-60 min -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K754 Asparaginase Activity Colorimetric/Fluorometric Assay Kit. K754-100 is the same size as the 100 test size of ab107922.
Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Asparaginase Positive Control 1 vial Aspartate Enzyme Mix 1 vial Aspartate Standard 1 x 0.1ml Assay Buffer IV 1 x 25ml Converter Enzyme XII 1 vial OxiRed Probe 1 x 0.2ml Asparaginase Substrate Mix 1 vial -
Research areas
Images
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Typical asparaginase standard calibration curve using colorimetric reading.
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Typical asparaginase standard calibration curve using fluorometric reading.
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Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.
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Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab107922 has been referenced in 5 publications.
- Biswas P et al. A Mouse Model with Ablated Asparaginase and Isoaspartyl Peptidase 1 (Asrgl1) Develops Early Onset Retinal Degeneration (RD) Recapitulating the Human Phenotype. Genes (Basel) 13:N/A (2022). PubMed: 36011372
- Lin HC et al. Diverse Enzymes With Industrial Applications in Four Thraustochytrid Genera. Front Microbiol 11:573907 (2020). PubMed: 33193181
- Kim K et al. Cell mass-dependent expression of an anticancer protein drug by tumor-targetedSalmonella. Oncotarget 9:8548-8559 (2018). PubMed: 29492216
- Biswas P et al. A missense mutation in ASRGL1 is involved in causing autosomal recessive retinal degeneration. Hum Mol Genet 25:2483-2497 (2016). PubMed: 27106100
- Kim K et al. L-Asparaginase delivered by Salmonella typhimurium suppresses solid tumors. Mol Ther Oncolytics 2:15007 (2015). PubMed: 27119104